Protein determination

The Kjeldahl method is a method for the fast quantitative determination of nitrogen in chemical substances developed by a Danish chemist Johan Kjeldahl in 1883. The Kjeldahl method of nitrogen analysis is the most accurate worldwide standard for calculating the protein content in a wide variety of materials ranging from human and animal food, beverages, grain, wastewater, soil and fossil fuels.

The method has been approved by various scientific associations including: AOAC International (formerly the Association of Official Analytical Chemists), United States Environmental Protection Agency, International Standards Organization, United States Department of Agriculture, and different Directives of European organizations.

The standard Kjeldahl method consists of three steps, commonly termed Digestion (Mineralization), Distillation, and Titration.

Method is based on the quantitative determination of nitrogen in chemical substances. The sample is first digested in strong sulphuric acid in the presence of a catalyst, which helps in the conversion of the amine nitrogen to ammonium ions, the organic material is destructed. The ammonium ions are then converted into ammonia gas by adding sodium hydroxide, heated and distilled. The ammonia gas is led into a trapping solution where it dissolves and becomes an ammonium ion once again. Finally, the amount of the ammonia that has been trapped is determined by titration with a standard solution, and a calculation made.
Despite the high complexity of the method, it has been recognized as a traditional arbitrage method. For time and the world do not stand still, our company has taken care of creation the equipment for laboratories to simplify the analysis that would be able to cope with this problem easily, quickly and safely.